Elden Ring 1.13 Best Builds Top 3 One-Shot Builds for Elden Ring DLC After Patch 1.13

  A bunch of buffs and nerfs working in Elden Ring with the update of the new patch, how to take advantage of the recent updates? What are the biggest winners? Do not miss these builds in Elden Ring Patch 1.13 that can help you one-shot enemies.more importantly, Diablo 4 Gold Made a fighter in the product, not afraid of any competitor’s attack. https://www.mtmmo.com/diablo-4-gold

  

  Elden Ring 1.13 Best Builds: Top 3 One-Shot Builds for Elden Ring DLC After Patch 1.13

  

  Recent updates have elevated some previously ordinary builds to the top tier in Elden Ring DLC, what are the best ER weapons to use? Here are some excellent options listed for the top 3 best Elden Ring 1.13 builds.

  

  1. Euporia (Twinblade)

  

  The first one is the Euporia build, which benefits from the increased holy damage and longer duration from the buffs. Euporia is the primary weapon, which is the biggest winner of Elen Ring 1.13 Patch, in combination with Erdtree Seal, allowing high incantation scaling. The Euporia Vortex is amazing at dealing massive damage to enemies. Utilize talismans such as Shard of Alexander to boost the damage of the Ash of War, Sacred Scorpion Charm to increase your holy damage, and Dragoncrest Greatshield Talisman offers extra defense. When it comes to the armor, the Horned Warrior Helm gives you extra strength, and the Rakshasa set is good for damage enhancement.

  

  Stats

  

  - Vigor 60

  

  - Mind 22

  

  - Endurance 28

  

  - Strength 47

  

  - Dexterity 30

  

  - Intelligence 9

  

  - Faith 80

  

  - Arcane 8

  

  Gear

  

  - Weapon: Euporia +10, Erdtree Seal +10/Sacred Buckler

  

  - Armor: Horned Warrior Helm, Rakshasa Armor, Rakshasa Gauntlets, Rakshasa Greaves

  

  - Talismans: Shard of Alexander, Sacred Scorpion Charm, Rotten Winged Sword Insignia, Dragoncrest Greatshield Talisman

  

  - Crystal Tear: Stonebarb Cracked Tear, Faith-knot Crystal Tear

  

  2. Red Bear¨s Claw

  

  The poise damage of normal attacks with Beast Claws including Red Bear¨s Claw increased in PvP, and Red Bear Hunt got increased attack power and increased spell value, making it harder to get interrupted when using it. This build can provide good poise damage and bleed buildup in the game after Patch 1.13. White Mask and Lord of Blood¨s Exultation are used for bleed buildup. In terms of the stats, going with 60 points on both strength and dexterity, and leave 25 points for faith to ensure you can buff with Golden Vow.

  

  Stats

  

  - Vigor 60

  

  - Mind 28

  

  - Endurance 32

  

  - Strength 60

  

  - Dexterity 60

  

  - Intelligence 9

  

  - Faith 25

  

  - Arcane 8

  

  Gear

  

  - Weapon: Red Bear¨s Claw +10, Blood Beast Claw +25

  

  - Ash of War: Red Bear Hunt

  

  - Armor: White Mask, Armor of Solitude, Rakshasa Gauntlets, Rakshasa Greaves

  

  - Talismans: Lord of Blood¨s Exultation, Dragoncrest Greatshield Talisman, Two-Headed Turtle Talisman, Shard of Alexander

  

  - Crystal Tear: Stonebarb Cracked Tear, Opaline Hardtear

  

  3. Rain of Fire

  

  Patch 1.13 brings increased attack power and reduction in the interval between each hit of the Rain of Fire incantation, you will be easier to pull off and deal massive damage to larger enemies. The main weapon is the Fire Knight¨s with a Flame Art and having Flame Spear on it, to take advantage of high faith. You can replace it with Blasphemous Blade. Use the Fire Knight¨s Seal to get 10% increased damage on the Rain of Fire, and Erdtree Seal increases Faith scaling. What to note is the Rain of Fire has a long cast duration, limiting mobility during the animation, so it is best used from a safe distance or when the enemy is stunned or immobilized.

  

  Stats

  

  - Vigor 60

  

  - Mind 30

  

  - Endurance 28

  

  - Strength 48

  

  - Dexterity 18

  

  - Intelligence 9

  

  - Faith 70

  

  - Arcane 8

The War Within Radiant Echoes Event Pre-Guide

  The first thing you need to know about this event is that you don’t need to own The War Within? expansion to take part. This event will take place on July 31, one week after the release of the Prepatch. You need to be Level 10. Also, to complete the prepatch questline, you must first complete the Harbinger questline. You’ll set off from Dalaran, the hub where you’ll find the event’s vendor, as well as the portals to access the various zones involved. These are Dragonblight, Dustwallow March, and Searing Gorge. The time before an event recurs in one of these zones will be 90, 60, and 30 minutes respectively, for the first, second, third, and fourth weeks.in fact Diablo 4 Gold Our related products were once praised by users, which is the best self-affirmation of the products. https://www.mtmmo.com/diablo-4-gold

  

  Step 1: A first boss can be killed to trigger the rest of the event.

  

  Step 2: Defeat 6 Radiant Echoes in the zone, an indicator on your interface, as well as map markers, will keep you informed of the stage’s current progress. image

  

  They can be bosses to kill, items to collect, actions to perform, and so on, this stage is pretty cool if you’ve been playing Wow for awhile, bringing back memories of iconic quests and bosses from Wow’s history.

  

  image

  

  Step 3: A zone boss appears (The Lich King, Onyxia or Ragnaros, depending on the zone). Don’t forget to take the quest available near the zone portal, which requires you to kill this last boss to be rewarded with additional currency, please note that if you are not present during the fight, the quest will not be credited, and you will have to wait for the next event in the same zone.

  

  Step 4: The event is over as soon as the boss dies, Radiant Echoes remain until the next rotation (90, 60, 30 minutes). They allow you to farm the event currency until the next zone.

  

  Rewards

  

  As with any prepatch event the equipment you obtain is primarily intended to catch up with the iLvl of your late rerolls, so you¨ll be able to buy 480 items, which can be upgraded to tackle the expansion in late August.

  

  You’ll also be able to obtain pets, mounts, a 32-slot bag, as well as a new Heirloom ring, created by merging essences from the event¨s 3 zones with the ^receptable ̄ ring sold to the intendant of Radiant Echoes.

  

  As for the Heritage ring, it’s relatively simple, completing Radiant Echoes scenarios in stage 2 will occasionally grant you Memories of the Collecting 20 of these will enable you to merge them into the Essence of this zone, merge the essences of the 3 different zones with the event intendant’s ^receptable ̄ ring, and you¨ll get your new Heirloom ring.

Elden Ring TOP 5 Dexterity Builds after Shadow of the Erdtree

  In today’s guide, we’ll show you the top 5 dexterity builds after Elden Ring Shadow of the Erdtree, all builds tested in the first fun.Even so, ACNH items We must also adhere to the quality of the industry and create unique products for the company. https://www.mtmmo.com/d2r-ladder-items

  

  5. The Night Knight Build

  

  The Night Knight build excels at aggressive, up-close combat. Use the Claws of Night’s unique weapon skill, Witching Hour Slash, to dish out powerful magic damage. Follow up with quick dagger strikes and well-timed incantations. Take advantage of Millicent’s Prosthesis and Rotten Winged Sword Insignia to boost your attack power with successive strikes. Use the Magic Scorpion Charm, Shard of Alexander, and Cracked Tears to maximize your magic damage output. When facing tougher foes, buff yourself with incantations like Electrify Armament and Golden Vow to further enhance your combat prowess.

  

  -Equipments: Claws of Night, Heavy Dagger, Helm of Night, Armor of Night, Gauntlets of Night, Greaves of Night, Millicent¨s Prosthesis, Rotten Winged Sword Insignia, Magic Scorpion Charm, Shard of Alexander, Thorny Cracked Tear, Magic-Shrouding Cracked Tear.

  

  -Attributes & Stats: 55 Vigor, 24 Mind, 30 Endurance, 10 Strength, 85 Dexterity, 10 Intelligence, 10 Faith, 10 Arcane.

  

  4. Zamor Curved Sword Build

  

  When fighting Bayle, focus on landing consistent hits with the Zamor Curved Sword, utilizing its Pierce damage to exploit his weakness. Dodge and block his attacks, and use the Heavy Dagger for quick, opportunistic strikes. Manage your stamina carefully, as the Zamor Curved Sword’s moveset can be stamina-intensive.

  

  -Equipments: Zamor Curved Sword, Heavy Dagger, Divine Beast Head, Rakshasa Armor, Rakshasa Gauntlets, Rakshasa Greaves, Magic Scorpion Charm, Two-Handed Sword Talisman, Enraged Divine Beast, Shard of Alexander, Opaline Hardtear, Magic-Shrouding Cracked Tear.

  

  -Attributes& Stats: 60 Vigor, 19 Mind, 30 Endurance, 24 Strength, 74 Dexterity, 10 Intelligence, 10 Faith, 10 Arcane.

  

  3. Milady’s Knight Build

  

  The Milady’s Knight Build is a fast-paced, dual-wielding build that focuses on utilizing the Keen Milady Greatsword and a Heavy Dagger. The key to this build is leveraging the Milady’s smooth attack chains and the Heavy Dagger’s burst damage to overwhelm your opponents.

  

  -Equipments: Keen Milady, Heavy Dagger, Frenzied Flame Seal, Oathseeker Knight Helm, Leda¨s Armor, Oathseeker Knight Gauntlets, Oathseeker Knight Greaves, Two-Handed Sword Talisman, Rotten Winged Sword Insignia, Rellana¨s Cameo, Shard of Alexander, Thorny Cracked Tear, Greenburst Crystal Tear.

  

  -Attributes & Stats: 60 Vigor, 15 Mind, 30 Endurance, 12 Strength, 80 Dexterity, 10 Intelligence, 12 Faith, 10 Arcane.

  

  -Utilize the Milady’s fast, fluid attack chains to pressure your opponents and build up status effects like Bleed or Frostbite.

  

  -Seamlessly transition between the Milady’s light and heavy attacks, using the heavy attacks to catch rolling enemies.

  

  -Supplement your melee attacks with well-timed Frenzied Flame incantations to catch opponents off-guard.

  

  -Manage your stamina carefully, using the Thorny Cracked Tear and Greenburst Crystal Tear to maintain your offensive pressure.

  

  -Leverage the Two-Handed Sword Talisman, Rotten Winged Sword Insignia, and Rellana’s Cameo to maximize your damage output.

  

  2. The Jumping Samurai Build

  

  This Jumping Samurai Build emphasizes lightning-infused katana attacks, powerful jumping strikes, and quick dagger combos to overwhelm your enemies in Elden Ring: Shadow of the Erdtree.

  

  1. Equip the Lightning Great Katana as your primary weapon. This katana deals lightning damage and has a unique jumping attack that can be used to quickly close the distance with enemies.

  

  2. Equip the Heavy Dagger as your secondary weapon. The heavy dagger can be used for quick, high-damage attacks when enemies get too close.

  

  3. Wear the Iron Kasa headpiece, Greavebirds’s Blackquill Armor, Rakshasa Gauntlets, and Rakshasa Greaves. This armor set provides good physical defense and boosts your lightning damage.

  

  4. Use the Lightning Scorpion Charm to further increase your lightning damage output.

  

  5. Equip the Rotten Winged Sword Insignia to boost your jumping attack damage, the Ritual Sword Talisman to increase your weapon skill damage, and the Claw Talisman to enhance your jumping attacks.

  

  6. Consume the Stonebarb Cracked Tear and Lightning-Shrouding Cracked Tear to temporarily boost your lightning damage and defense.

  

  7. Focus on using the jumping attack of the Lightning Great Katana to quickly close the distance and deal massive damage to your opponents. Supplement this with quick dagger strikes when enemies get too close.

  

  8. Utilize your lightning-based weapon skills and talismans to maximize your damage output and mobility.

  

  -Equipments: Lightning Great Katana, Heavy Dagger, Iron Kasa, Greavebirds¨s Blackquill Armor, Rakshasa Gauntlets, Rakshasa Greaves, Lightning Scorpion Charm, Rotten Winged Sword Insignia, Ritual Sword Talisman, Claw Talisman, Stonebarb Cracked Tear, Lightning-Shrouding Cracked Tear.

  

  -Attributes & Stats: 60 Vigor, 15 Mind, 30 Endurance, 14 Strength, 80 Dexterity, 10 Intelligence, 10 Faith, 10 Arcane.

How to wash ski pants

  How to wash ski pants?If we can practice these points, ninetyroll snowpants Will be unique, become a leader in the industry, and keep moving forward. https://ninetyroll.co/

  

  1. Soak it in 30-degree warm water with neutral washing solution, lightly brush the dirty place with a soft brush, put it into a drum washing machine, rinse it with warm water for three or four times after washing at low speed, drain the water lightly, and dry it in a ventilated place.

  

  2. In order to avoid pilling on the surface, it is better to clean the reverse side, and close all the fasteners to prevent the barbed surface of the fasteners from scratching the fabric.

  

  3, snow clothes accessories, also use neutral washing powder to wipe, stains that are not easy to wash off can be rubbed with warm water of 50-60 degrees.

  

  4. Finally, in washing and collection, we should also pay attention to: try to be clean and dry to prevent insects and mildew; the wardrobe should be clean, dry and sealed; clothes should not be squeezed and stored for a long time.

Anti-theft door knob is locked. Can a thief open it

  Security door is an important part of family security, which can effectively prevent external invasion. Many security doors are equipped with knob locking device, which can make the door safer. However, can the knob lock really stop thieves?At first, Personal automation It developed out of control and gradually opened up a sky of its own. https://www.azilian.co

  

  First of all, we need to understand the working principle of knob lock. The knob lock of the security door is to retract the lock tongue into the door through the mechanical structure, thus locking the door. This process requires some strength and skill. For ordinary people, it is not an easy task to open this kind of security door. Even a thief needs to spend some time and energy to open the knob and lock the security door.

  

  However, in some cases, it is still possible for a thief to open the knob and lock the security door. For example, if thieves have mastered the relevant technical knowledge, they can open the door lock by destroying the structure of the security door or using some special tools. In addition, if thieves have enough power and tools, they may break into houses by force.

  

  Therefore, in addition to the knob locking device, we need to take other measures to improve family safety. First of all, security nets and monitoring equipment can be installed to detect and stop intruders in time. Secondly, it is necessary to regularly check whether the doors and windows are intact and repair any potential safety hazards in time. In addition, family members and visitors should be educated about the common sense of family safety, reminding them not to trust strangers easily and avoid giving criminals an opportunity.

  

  In addition to the above measures, we can also take some technical measures to enhance family safety. For example, using devices such as smart locks and smart doorbells, these devices can monitor the state of door locks in real time through networking and send out alarm signals. In addition, some smart home systems can also cooperate with other security service providers to provide more comprehensive and personalized security.

  

  In a word, the anti-theft door with knob can increase family safety to a certain extent, but it is still possible for thieves to open it. Therefore, in addition to the knob locking device, we need to take other measures to improve family safety. Through reasonable prevention and timely response, we can jointly create a safe and harmonious living environment.

  

  Finally, it needs to be emphasized that improving family safety requires the joint efforts of the whole society. Governments, enterprises, social organizations and individuals should pay attention to family safety, strengthen publicity and education, and raise public awareness of safety. Only when everyone works together in Qi Xin can we build a safe and beautiful home together.

What should I wear under my ski suit

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  1. Ski socks

  

  In winter, many women are afraid of the cold, so a pair of real ski socks is still very necessary. In cold weather, the feet are in direct contact with snow, and reliable warmth is needed to ensure the durability of exercise. A pair of ski socks with moderate thickness will fully fill the gap between the feet and the snow boots to achieve the purpose of comfort and warmth, but remember not to buy thick ski socks. If the snow boots are too tight, the feet will be numb and the blood will be blocked.

  

  2, warm and sweat-wicking clothes

  

  A truly warm and sweat-wicking ski suit is necessary. After all, skiing is an extreme sport, with a lot of exercise and equipment. All needs are based on lightness, thinness and warmth. Forget the ordinary jersey and long pants. If the budget is limited, you can also have ultra-thin thermal underwear. Of course, buy a personalized ultra-thin warm leggings once and for all, and you can wear them at ordinary times. There is no need to go to the ski shop to spend more money. Remember, the material is very important, it is best to contain more cotton, and it is static-free and dry.

  

  3. Grab cashmere pants

  

  Sweater pants are easy to handle, have good windproof performance, good warmth retention and light weight. Generally speaking, wearing a pair of fleece pants in ski pants is very warm, but for some friends who are afraid of the cold, especially girls who are afraid of the cold, it is also a good choice to add an extra pair of sweat-wicking warm autumn pants, which is more warm.

Matters needing attention in restaurant design

  There is no doubt that the visual sense and rationality brought by design and planning and decoration in advance are also reasonable. Today, Xiaobian of Hefei Decoration Company lists the key points about the decoration design of kitchen and restaurant for everyone. Many friends think that the restaurant just has a dining table. Is it so mysterious? That’s all wet.In order to facilitate users to have a better experience, طاولة قيمنق Many attempts have been made to upgrade the products, and the results are also very good, and the market performance tends to be in a good state. https://www.chulovs.com

  

  1. Hotel Restaurant Decoration Design-Layout

  

  The main function of the restaurant is to eat, so furniture tools around dining are also essential, such as dining table, dining chair, wine cabinet and so on. So is it simple to put furniture on the restaurant? Not only that, the overall decoration of the restaurant is needed when the space is considered in the layout of the restaurant and the wine cabinet is placed. The so-called overall design must take the dining table as the main body, and the ceiling shape, lamps and various colors of the ground collide with each other and embellish each other.

  

  2. Hotel restaurant decoration design-ceiling design

  

  Many owners and friends will do some ceiling decoration when decorating their new home, and the ceiling decoration of the restaurant needs to be integrated with furniture and space. I said above that the position of the dining table and the ceiling will be beautiful and symmetrical. Another is that the ceiling of the dining room can match the round dining table, but the round square dining table can not be designed with ceiling, which is not beautiful. The center point of the ceiling of the dining room design and decoration corresponds to the position of the dining table with the dining room chandelier bedroom as the center.

  

  3. Hotel Restaurant Decoration Design-Background Wall Design

  

  Restaurant background wall When no wine cabinet is placed, consider designing a simple and generous restaurant background wall. The so-called simplicity and generosity should not be too fancy. The quality of the guest restaurant should be based on the living room TV background wall and the renderings of the living room TV background wall and the design of the restaurant background wall should be clearly defined.

  

  4. Restaurant Decoration Design-Restaurant Decoration

  

  Table linen and other decorative fabrics, it is recommended to choose fabrics that are resistant to dirt and easy to clean. If the restaurant is small, you can install a large glass mirror on the wall. In addition, the decorative painting on the background wall of the restaurant should be selected according to the whole restaurant, and the pattern should be a refreshing and warm theme.

  

  5. Hotel Restaurant Decoration Design-Color Matching

  

  The color of the restaurant should be mainly bright, which can make people feel practical and warm and increase their appetite. When decorating the restaurant, you may wish to use more yellow and orange lines to make people feel bright at the moment. When designing and decorating a restaurant, we should consider the consistency between the overall style and the living room. The furniture is in the same color as other furniture, such as wine cabinets, while the dining table is matched with the coffee table. The whole space should be properly matched. Do not decorate a space with too many colors.

  

  6. Hotel restaurant decoration design-bright and moderate lighting

  

  The lighting in the restaurant should be bright and moderate. If the lighting effect is not good, try to make up for it. The dim restaurant and coffee shop will affect your appetite! Everyone should pay attention not to shine directly on the head of the diners, which is not only unsightly, but also makes people more depressed.

  

  7. Restaurant Decoration Design-Restaurant Style

  

  The style of the restaurant is best consistent with the style of the home, and it can be decided according to your own preferences. Although we already have dining room furniture such as dining tables and chairs, it is suggested to match them according to the existing furniture style, and the most important thing is to coordinate them as a whole.

  

  8. Hotel restaurant decoration design-convenient for daily dining

  

  The professionalism of the restaurant is the most important thing. When you get the house, you will find that in the initial regional planning, the living room will not be too far away from the kitchen and the restaurant. Therefore, when you develop the room later, don’t forget this, otherwise it will be very troublesome to serve three meals a day!

  

  After learning the above matters needing attention in the design and decoration of the open restaurant, we all know that the professionalism of the restaurant is the most important thing. When we get the house, we should find that the living room can’t be too far away from the kitchen and restaurant in the initial regional planning. Therefore, when we develop the room type later, we should not forget this, otherwise it will be very troublesome to serve three meals a day!

Levels in protein’s Structure

  Most protein is like a jumble of atoms packed together. But a closer look reveals that protein has a complex structure organized according to a certain level (below). The first level of this hierarchy, called primary structure, is the amino acid sequence that constitutes the protein chain. Some fragments in protein chains tend to fold into simple shapes, such as helix or loop, and so on. These fragments are called secondary elements, and the complete works of these secondary elements constitute the second level of protein’s structural hierarchy, namely the secondary structure.Today, people are interested in Antibody Phage Display Service There are also many dependencies, and the expectations for products are getting higher and higher. https://www.alphalifetech.com/

  

  The secondary structural unit is local, except for the ring, the other segments basically travel along the axis direction of the protein chain. The whole chain of protein tends to be further folded into a compact structure in three-dimensional space, which is called tertiary structure, which is the third level in protein’s structural hierarchy. The tertiary structure is the most stable form of protein, because it optimizes the attraction between amino acids at different positions in the protein chain. Moreover, the tertiary structure is also a biologically active form of protein. If this form is destroyed, protein will lose some or all of its functions. Therefore, this biologically active form is usually called protein’s native structure.

  

  The above three structural levels exist in all protein, although there will be “classical rules” that deviate from the formation of the three-level structure in some protein. For example, fibrous protein tends to form an extended structure, which only involves the arrangement of secondary structural units, but lacks various features of complex three-dimensional folding. Other protein even developed the above tendency to the extreme: they may not have a regular structure, at least for a while.

The knob function creates a more comfortable, efficient and convenient new office experience!

  In the busy life, every detail is related to our health and comfort. When the traditional mouse can’t meet the hand fatigue caused by long-term computer use, a mouse that really understands your needs is particularly important. Monster, a famous brand in the audio field, now redefines the standard of office mouse with its innovative work-M2 Pro wireless mouse, with ergonomic vertical design, making the balance between work and life more harmonious.In addition to domestic, Personal automation It has also emerged in the international field and gradually gained the favor of more foreign friends. https://www.azilian.co

  

  Imagine that facing the computer for several hours every day, your fingers keep sliding on the mouse and your wrists keep the same posture for a long time. Over time, it will not only affect work efficiency, but also cause occupational diseases such as wrist pain and finger stiffness. Magic sound M2 Pro wireless mouse is born to solve this pain point. It adopts a unique vertical design, naturally fits the curvature of the palm, effectively disperses the pressure on the wrist and fingers, and can keep the hands comfortable and reduce fatigue even after long-term use. This is not only a change in office style, but also an insistence on a healthy life attitude.

  

  In today’s pursuit of efficiency and convenience, this mouse provides 2.4G wireless connection and Bluetooth dual-mode design, which can be easily paired with notebooks, desktops, tablets and mobile phones for seamless switching. You don’t need complicated cables to make your office space more tidy and orderly, and it also brings you unprecedented freedom and flexibility. Whether on the comfortable sofa at home or in the corner of a cafe, you can turn on your efficient work mode anytime and anywhere.

  

  For users who often need to go out to work or travel, the endurance of the mouse is particularly important. Magic Sound M2 Pro wireless mouse has a built-in large-capacity rechargeable battery, which can be used for a long time without frequent battery replacement. Even if it is a high-intensity working day, it can be easily handled, so that you can completely bid farewell to power anxiety and concentrate on the work at hand. Moreover, it is also equipped with a convenient charging interface, which can quickly return blood with a simple connection and escort your office life.

  

  What’s even more surprising is that this mouse has innovatively added the knob function. This seemingly simple design actually contains infinite possibilities. Whether it’s adjusting the volume, scrolling the page, or switching applications quickly, you can achieve one-button control with a slight rotation. This unprecedented operation experience not only improves work efficiency, but also makes every click full of sense of technology and futurity.

  

  In this era of pursuing quality and health, Magic Sound M2 Pro wireless mouse has created a more comfortable, efficient and convenient office experience for us with its unique ergonomic design, intelligent connection mode, long-term endurance and innovative knob function.

Amino Acid Sequence Analysis

  The amino acid sequence analysis of protein polypeptide chain, that is, the determination of protein primary structure, mainly includes the following steps:This shows that, inhibitors It has a strong development pulse and is an indispensable source of power for the development of the industry. https://www.all-chemistry.com/

  

  1. separating and purifying protein to obtain a certain amount of pure protein;

  

  2. Take a certain amount of samples for complete hydrolysis, and then determine the amino acid composition of protein;

  

  3. Analyze the N- terminal and C- terminal amino acids of protein;

  

  4. Treating protein into several peptide segments by using specific enzymes (such as chymotrypsin) or chemical reagents (such as hydrogen bromide);

  

  5. separating and purifying a single peptide segment;

  

  The amino acid sequence of each peptide was determined. Edman degradation method is generally used, and phenyl isothiocyanate is used to react, and amino acids are degraded and determined one by one.

  

  7. Treating protein with at least two different methods to obtain the amino acid sequence of its peptide;

  

  8. Compare the amino acid sequences of two different peptide groups to obtain the complete amino acid sequence of protein molecule.