Protein, the source of life, how much do you know? Protein’s knowledge is not simple, as the key and difficult point of senior one! Today, I will take you to find out and unlock the mystery of protein!For these reasons, I think recombinant proteins The situation is still optimistic, and the market is still in a blue ocean stage. https://www.alphalifetech.com/

　　Protein is the basic substance of cells and tissues. Without it, there would be no life. They are composed of amino acids, and through different sequences and structures, they form an ever-changing protein. This is like Lego of life. Every amino acid is a building block, which is combined to construct the diversity of life.

　　Protein has various functions, from structural support to catalytic reaction, from transporting substances to signal transmission, and they are indispensable roles in cells. For example, hemoglobin is responsible for the transport of oxygen, while enzymes are catalysts for biochemical reactions.

　　The synthesis process of protein is also very interesting. The process from transcription of DNA into mRNA to translation into protein is called the Central Rule. It reveals the flow direction of genetic information and is the key to understanding life phenomena.

　　The structure and function of protein are closely related. The primary structure is amino acid sequence, the secondary structure is partial folding, the tertiary structure is integral folding, and the quaternary structure is the combination of multiple subunits. These structural levels determine the function and stability of protein.

　　Protein is the cornerstone of life. To understand them is to understand the mystery of life. Through today’s study, I hope you can have a deeper understanding of protein and lay a solid foundation for senior high school biology!

　　Focus on glycolysis, aerobic oxidation of sugar, pentose phosphate bypass, gluconeogenesis, synthesis of ketone bodies, cholesterol and phospholipids, respiratory chain, oxidative phosphorylation, special amino acid metabolites, one carbon unit metabolism, purine and pyrimidine nucleotide synthesis raw materials and catabolites, and substance metabolism.Without exception, small molecules Our customers are willing to purchase their products, because high quality is the concept of their products. https://www.all-chemistry.com/

　　The chapter on sugar metabolism is the focus of the exam, so we should master it comprehensively. The chemical reaction formula of sugar metabolism is complicated, and the focus of the annual examination is basically on the reaction site, key enzymes and regulation, energy production and the relationship between important substances. Glycolysis, gluconeogenesis and aerobic oxidation of sugar are all contents that must be familiar with. The synthesis of ketone bodies and cholesterol in lipid metabolism, pay attention to the difference between them. Ketones are synthesized in the liver and utilized outside the liver, while fats are synthesized in the liver and stored outside the liver. Fat synthesis, ketone body synthesis and cholesterol synthesis are the raw materials of acetyl-CoA synthesis. Synthesis and decomposition of fatty acid, the main material of fatty acid synthesis is acetyl CoA; Under the condition of sufficient oxygen supply, fatty acid is decomposed into CO2 and water in the body, releasing a lot of energy, which is the main form of fatty acid catabolism in the body. Phospholipid synthesis. Differentiation of several blood lipids.

　　Composition of respiratory chain and influencing factors of oxidative phosphorylation. Urea synthesis-ornithine cycle. One carbon unit’s metabolism is often tested, but it is easy to remember as long as you grasp the core content. It comes from one carbon unit-Sisegan, and is transported by one carbon unit-tetrahydrofolate. One carbon unit plays an important role in nucleic acid biosynthesis as a raw material for purine and pyrimidine synthesis, connecting amino acids and nucleotides. Comparison of nucleotide synthesis and decomposition between purine and pyrimidine. The changes in this year’s syllabus are: the deamination of amino acids (oxidative deamination, transamination and combined deamination) is changed to the general metabolism of amino acids (degradation of protein in vivo, oxidative deamination, transamination and combined deamination), which should be reviewed.

　　Protein is a substance with a certain spatial structure, which is formed by the zigzag folding of polypeptide chains composed of amino acids in the form of dehydration and condensation. Protein must contain carbon, hydrogen, oxygen and nitrogen.If you want to make a big difference in the market, Antibody Phage Display Service It is necessary to intensify the upgrading of products on the original basis in order to meet the consumption needs of consumers. https://www.alphalifetech.com/

　　Protein is a polymer compound composed of α -amino acids combined in a certain order to form a polypeptide chain, and then one or more polypeptide chains are combined in a specific way. Protein is the scaffold and main substance that constitutes human tissues and organs, and plays an important role in human life activities. It can be said that there would be no life activities without protein.

　　Number of atoms

　　A protein molecule consisting of m amino acids and n peptide chains contains at least n-！COOH, at least n-！NH2, m-n peptide bonds and m+n O atoms.

　　molecular mass

　　Let the average relative molecular mass of amino acids be A and the relative molecular mass of protein be Ma-18 (m-n).

　　Gene control

　　Nucleotide 6 in the gene

　　Nucleotide 3 in messenger RNA

　　Amino acid 1 in protein

　　Focus on glycolysis, aerobic oxidation of sugar, pentose phosphate bypass, gluconeogenesis, synthesis of ketone bodies, cholesterol and phospholipids, respiratory chain, oxidative phosphorylation, special amino acid metabolites, one carbon unit metabolism, purine and pyrimidine nucleotide synthesis raw materials and catabolites, and substance metabolism.As an important brand soul of the company, inhibitors Has outstanding performance, through the market test, still has a strong development trend. https://www.all-chemistry.com/

　　The chapter on sugar metabolism is the focus of the exam, so we should master it comprehensively. The chemical reaction formula of sugar metabolism is complicated, and the focus of the annual examination is basically on the reaction site, key enzymes and regulation, energy production and the relationship between important substances. Glycolysis, gluconeogenesis and aerobic oxidation of sugar are all contents that must be familiar with. The synthesis of ketone bodies and cholesterol in lipid metabolism, pay attention to the difference between them. Ketones are synthesized in the liver and utilized outside the liver, while fats are synthesized in the liver and stored outside the liver. Fat synthesis, ketone body synthesis and cholesterol synthesis are the raw materials of acetyl-CoA synthesis. Synthesis and decomposition of fatty acid, the main material of fatty acid synthesis is acetyl CoA; Under the condition of sufficient oxygen supply, fatty acid is decomposed into CO2 and water in the body, releasing a lot of energy, which is the main form of fatty acid catabolism in the body. Phospholipid synthesis. Differentiation of several blood lipids.

　　Composition of respiratory chain and influencing factors of oxidative phosphorylation. Urea synthesis-ornithine cycle. One carbon unit’s metabolism is often tested, but it is easy to remember as long as you grasp the core content. It comes from one carbon unit-Sisegan, and is transported by one carbon unit-tetrahydrofolate. One carbon unit plays an important role in nucleic acid biosynthesis as a raw material for purine and pyrimidine synthesis, connecting amino acids and nucleotides. Comparison of nucleotide synthesis and decomposition between purine and pyrimidine. The changes in this year’s syllabus are: the deamination of amino acids (oxidative deamination, transamination and combined deamination) is changed to the general metabolism of amino acids (degradation of protein in vivo, oxidative deamination, transamination and combined deamination), which should be reviewed.

　　Protein is a complex organic polymer compound composed of α -amino acids combined in a certain order to form a polypeptide chain, and then one or more polypeptide chains are combined in a specific way. Amino acids are the basic units of protein, and they are linked into peptide chains through dehydration and condensation. Each polypeptide chain has twenty to hundreds of amino acid residues (-R); Various amino acid residues are arranged in a certain order.As a representative of the industry, Antibody Phage Display Service It is necessary to set a certain example for peers and lead the way in product quality. https://www.alphalifetech.com/

　　the composition characteristics of protein

　　The amino acid sequence of protein is encoded by the corresponding gene. In addition to the 20 basic amino acids encoded by the genetic code, in protein, some amino acid residues can be modified after translation to change their chemical structure, thus activating or regulating protein. Multiple protein can form a stable protein complex together, often by combining together, and fold or spiral to form a certain spatial structure, thus playing a specific function. The organelles of synthetic peptides are ribosomes on rough endoplasmic reticulum in cytoplasm. The difference of protein lies in the variety, number, arrangement order of amino acids and the spatial structure of peptide chains.

　　Protein is composed of C (carbon), H (hydrogen), O (oxygen) and N (nitrogen), and generally protein may also contain P (phosphorus), S (sulfur), Fe (iron), Zn (zinc), Cu (copper), B (boron), Mn (manganese) and I (iodine).

　　The composition percentage of these elements in protein is about: 50% carbon, 7% hydrogen, 23% oxygen, 16% nitrogen, 0-3% sulfur and other trace amounts.

　　All protein contains N element, and the nitrogen content of various protein is very close, with an average of 16%;

　　Every 1g of N in any biological sample means that there is about 100/16=6.25g protein, and 6.25 is often called protein constant.

　　The reason why people live tired, is not fit the shelf, tear can not open face, untied plot (the reason is very simple)Doing these simple things can also make pu-erh tea Sowing high-quality genes will eventually grow into towering trees and become the leader in the industry.

　　Your life needs to understand the stand in their own position, not everyone is in line with your appetite; standing in other positions, you can also to all the appetite? (Tolerance of others)

　　People who can simplify complex problems are talented people, and it is silly to complicate simple problems. (People are often willing to be a fool)

　　A woman most admired men talented, most hated men are too stingy, most afraid of men do not live up to expectations. (Men afraid of the old woman angry)

　　Itching sometimes worse than pain, hate is often easier than love (paradox of life)

　　The opportunity for everyone is equal, you can not shoot the chance, but the opportunity comes when you can not do without bullets (doing business is to do this)

　　Love is like a smallpox, and each of us has to go through it once, and, as with smallpox, we will only get once in a lifetime. You never have to worry about getting a second time.

　　Ancient love stories and more contemporary love accident (different times).

　　A person who does not make mistakes usually can not accomplish anything, and there are no shortcomings, often with little merit (Lincoln’s experience).

　　Businessmen are “people who can talk,” businessmen are “people who can give birth to ideas.” (Alternative explanation)

　　There are seven virtues: First, do not get the ax, the second is not to interrupt other words, the third is not anxious for success, four questions should be targeted, five answers to meet the reasonable, six is to have a beginning and end, seven is to Based on reality. (Ancient Jewish Proverbs).

　　Unit to work overtime every day; home never stick; mobile phone home to close; SMS reading on the delete; go to bed snoring shaking; underwear often wear back. (With an affair of the six symptoms).

　　Do what you think can not do (how much heart, how far the road).

　　The heart is a two-bedroom house, one is suffering, one is happy. People can not laugh too loud, or laughter will wake up next to the pain. (To avoid very happy).

　　If you marry a wise and virtuous wife, you will be happy; if you marry a frivolous bitch, you will become a philosopher. (Marriage useful).

　　Can not tell, can not cry, is the most bitter (life reflected).

　　Where to fall, just lie down (some pessimists).

　　The real question is not whether the spouse is the best person, but whether you are trying to be the best person for your spouse (the Happy Marriage Bible).

　　The only advantage of telling the truth is that you do not have to remember what you said (Quotations User).

　　You can offend busy people, because they do not have time to care about you; you must not offend idlers, because they have time to deal with you. (Do not offend idlers).

　　If you can not change your face, then you change your face (not beautiful, it can be very cute).

　　Than bathing, washing the sauna is more important is the brainwashing, bath does not wash will be distributed odor, brain wash will be eliminated by the times (we often forget this).

　　Do not believe your memories, the person inside, not necessarily the same miss you (with memories of the road, rather than holding the memories of life).

　　There should be a better way to start a new day than waking up every morning (office worker philosopher).

　　Toad want to eat swan, good kind, small things with ambition (to educate the next generation).

　　Rich does not have much, but less luxury. (New view of wealth).

　　Life is a fleeting moment in the river of time, mankind do everything possible to seek forever and everlasting, but did not think, in fact, as long as the pace of their own slow down mentality flat, live in the present, then life will naturally become long and distant .In some cases, pu-erh tea The advantages will become more and more obvious, and it will be able to develop indomitable after market tests.

　　When I did not write a magazine before, I did not know that the time had come in advance. A draft of March or even April has been prepared in January. In the draft of the forum while watching the side gripped, his year has not yet ended, did not make a point, there people work has been a year, almost equal to the end. Years here, was actually can not wait to catch up with the speed of flight, how to make people panic?

　　The length of time is defined by man himself. 365 days a year, divided into four seasons, twelve months. Here, people always catch up with me like the game, to see who can one day into a day. Our ancestors know, if the decade into a year, we will so impatient it?

　　Days not used, can only be used to recover. Self has no intention to enter this door, it fell into such a turbulent swirl. Calendars and clocks on the numbers can only represent the past, because today to do, is already the future. Obviously is the spring, but the men have been doing non-stop fall thing.

　　There is nothing worthy of gratification, no reason for youth just a short length of a bit like others. What is the use of walking in front, time falls behind, urging the old soul and face it in vain.

　　The fast pace of modern people, nothing more than to speed up the clock of life, their own pressure and persecution of a way, only to more fully squeeze the juice of life to obtain value and fame. But I see it is faster and more leave no room for the consumption and flight. How, a ton of gold can be exchanged for even a second time?

　　Americans always run in front of the world, even spend today are tomorrow, the result? The financial crisis has finally shattered this myth of early consumption, punish all activities contrary to the laws of nature.

　　Beijing, then, a little leisurely. People live I, a spring vegetation, how short and beautiful time. If you can walk, why should anxious to run, miss the scenery all the way? Note that the front is not another spring, but has been waiting for you to close the end of life.

　　Tomorrow’s thing is done today, and next year’s event is finished this year. So ahead of the outstanding achievements and efforts, but is a result of the advance of life.

　　What is a rivers and lakes years reminder, juvenile children rivers and lakes old? This is the helplessness of life rather than enjoyment.

　　The first is “poverty”know pu-erh tea Our growth has to go through many hardships, but entrepreneurs are never afraid and boldly move forward.

　　Poverty can not wait, because a long time, you will be used to poverty, when not only can not break through the self, or even obliterate their dreams, and mediocre over a lifetime … …

　　The second is “dream”

　　Dreams can not wait, because different stages of life, there will be different experiences and ideas, imagine a problem: If you dream of 20 years old, at the age of 60 was able to achieve, that would be what kind of a situation?

　　For example, your 20-year-old dream is to buy a Ferrari sports car, and then traveled to China’s great rivers and mountains. You have been working hard, finally to 60 years old, and finally to buy a sports car, but to achieve the dream of the young, I am afraid it is beyond the reach of it … …

　　The third is “family”

　　Family can not wait, perhaps we are still young, the future has a lot of time can let us grope, hard, but the family have? They still have time for us to succeed? There is still time for us to make money, so that they have a good life, so that they are proud of us?

　　Trees for static and wind, the child wants to support and pro-not wait. This is a lot of people’s pain, but also a lot of people a lifetime of regret!

　　Bed is probably the most consumption life time place! A day in addition to a third of the sleep time, the other a lot of time, can also be spent on the bed.More importantly, put pu-erh tea It is imperative for us to make thorough analysis and maximize its social function.

　　Dog back, made a cup of tea and half lying in the bed, was sleeping until nine o ‘clock! Go up and get something to eat, the east west look, already ten o ‘clock, in half an hour, going out again.

　　Back on the playground, the ongoing autumn sports meeting of rehearsal. Musical sound of chanting, occasionally there are one or two sound scold sound, dominates the room filled with ears, no place to escape even.

　　Person, want to quiet for a while now, it’s too difficult. Have a computer, television, mobile phone at home. These things no matter use, is usually open. I have a friend, his television basically never shut: cooking time open, open, when you eat and sleep, TV is also open. Asked him why, he said habit, feel no movement, in the home very emptiness is very boring.

　　Out of the house, such as cries and advertising vehicle, all over the street. Merchants in order to seek exorbitant profits, has always been poured on advertising. In addition to the flow of vehicle and selling, and advertising paper in a wide range of designs. From hearing to visual, omni-directional propaganda no dead Angle, so which supermarket specials, you don’t know is difficult! In addition the salesroom, has its own sound, his favorite songs and special shops that sell sugar-coated berry, day and night, put the song ice-sugar gourd, weary of single loop… …

　　So, now the people want to quiet, to have deep spiritual practice. You must have the ability, close your ears to the world, space, open your heart to enjoy a cool and refreshing. Just such an opportunity, but also not much. Such and such things, always copy your heart very full is a mess. Many want to have a heart quiet ethereal! Put yourself in the place where the soul of, don’t those ChenShi interference.

　　All the fruit in the home, all have no, but how to don’t want to go out to buy. Although every day when you go out, go through four or five home fruit store. Not forget to take money, is to bring the money and forget to buy it. Home is always open the door of that a moment, before crashing. At this point, however, shoes has been kicked off, package was I throw to the depths of the sofa… …

　　And so next time, may is so every time.

　　Used to open the computer, the trouble to listen to the shaoxing opera. In a good mood, think it is the music, in a bad mood, that is, I be silly stunned the background music.

　　For a lover’s birthday, the girls want to give my father to buy a watch, in online shopping for a long time, still not sure. Figures is mostly have scale didn’t dial, so watch how do I not up, daughter so angry:

　　”Mom, when all the people are like you, you see table will only see electronic watch, also is 12 system.”

　　”Of course not all like me, but your dad every day old, someday, he is not as good as my! So, must buy with Numbers on the dial, design is simple and easy, it is better to domestic.”

　　”Domestic mechanical or quartz? What brand do you want?”

　　”To quartz, need not everyday winding, brand to the seagull.”

　　”Gull? Never heard of.”

　　”The Internet, as a Chinese, incredibly don’t know the seagull watch?”

　　A daughter called me again:

　　”Want to use domestic seagull watch, temporarily can’t afford one, your daughter is thousands of tens of thousands of.”

　　Then, my daughter on QQ, send me a link, I open on see, isn’t it? Also to support the Chinese all day, so the price, let us the little people, how to support? A newlywed, bought a watch the seagulls, less than 200 dollars.

　　Love came back from work, said to him. See I wasn’t impressed, he looked at me and said:

　　”You are less than 200 pieces, I was monthly salary less than 120 yuan! For more than a month of wages, or to the present, not thousands of useful!”

　　”Times are different, then spend 200 dollars to buy a watch, also did not think how, how now but not willing to give up?”

　　”Don’t think that way, that was didn’t spend your money.”

　　”You don’t say you bought me a watch, to now you still love dearly!”

　　Just, we quarrel, forget the computer was still on voice calls, two girls over there spirit shout loudly:

　　”How noisy again? Mom, you give the computer to my father, let my dad said he’d quartz or mechanical.” , I put my computer to lover.

　　”I want to mechanical, don’t be too high price, didn’t I give you remit money.”

　　”Dial? Digital or graduated? Color should be black and white or grey days?”

　　”Digital calibration, color should be black and white.”

　　”Oh, is it not a watch? Things are!” I can’t help by chimed in.

　　”Dad, are you sure?”

　　”Sure, your mother rush to surf the Internet!”

　　”Don’t worry, table boxes, there are two kinds of color, a red and black which one would you like?”

　　”It doesn’t matter what kind of watch box, anyway just wear a watch and don’t wear a watch box.” And I say a word of mouth.

　　”Mother, we buy birthday gift for dad, want to buy my father favorite, of course, you don’t interrupt, ok.”

　　”What color do you like table box, what colour will! Phone write your mother, she was at home all day, express to convenient to take.”

　　Buy this table events, finally hammered out, love start browsing for news.

　　”Take headphones to you, I want to quiet for a while.”

　　”Want to quiet, what did you do for dinner?” Yeah, just and daughter discuss watch things, how to forget to do dinner?

　　You see, this want to quiet for a moment, really difficult! You can close the earth is the ear, you don’t shut the appetite of the world! Darling still cooking go to! Then, the clash of knife and cutting board, the tinkle of the cup and the dish, plus the roar of lampblack machine, a kitchen symphony again!

　　”Shut the kitchen door, noisy dead.” Lover to roar, I closed the door of the kitchen. Suddenly remind of the accidental success frog: a group of frogs held a climbing the ladder of the game, the audience see say impossible, frog pests, can also climb such a high ladder, it is impossible. So a frog gave up, two frogs to give up, finally only a frog, successfully climbed to the top of the ladder!

　　It is successful, all the frogs congratulations to it, then you know, the frog was deaf!

　　Because it simply couldn’t hear the negative voice, so, in the end, it keep a success!

　　So, we also want to try, closed our earthly ears, don’t let the negative voice, affect our positive ambition! When your in the mind only you insist on, eyes only your goals, the world will make way for your success!

　　Bed is probably the most consumption life time place! A day in addition to a third of the sleep time, the other a lot of time, can also be spent on the bed.After that, pu-erh tea Not afraid of the future, dare to fight and fight, and won applause again and again in the market.

　　Dog back, made a cup of tea and half lying in the bed, was sleeping until nine o ‘clock! Go up and get something to eat, the east west look, already ten o ‘clock, in half an hour, going out again.

　　Back on the playground, the ongoing autumn sports meeting of rehearsal. Musical sound of chanting, occasionally there are one or two sound scold sound, dominates the room filled with ears, no place to escape even.

　　Person, want to quiet for a while now, it’s too difficult. Have a computer, television, mobile phone at home. These things no matter use, is usually open. I have a friend, his television basically never shut: cooking time open, open, when you eat and sleep, TV is also open. Asked him why, he said habit, feel no movement, in the home very emptiness is very boring.

　　Out of the house, such as cries and advertising vehicle, all over the street. Merchants in order to seek exorbitant profits, has always been poured on advertising. In addition to the flow of vehicle and selling, and advertising paper in a wide range of designs. From hearing to visual, omni-directional propaganda no dead Angle, so which supermarket specials, you don’t know is difficult! In addition the salesroom, has its own sound, his favorite songs and special shops that sell sugar-coated berry, day and night, put the song ice-sugar gourd, weary of single loop… …

　　So, now the people want to quiet, to have deep spiritual practice. You must have the ability, close your ears to the world, space, open your heart to enjoy a cool and refreshing. Just such an opportunity, but also not much. Such and such things, always copy your heart very full is a mess. Many want to have a heart quiet ethereal! Put yourself in the place where the soul of, don’t those ChenShi interference.

　　All the fruit in the home, all have no, but how to don’t want to go out to buy. Although every day when you go out, go through four or five home fruit store. Not forget to take money, is to bring the money and forget to buy it. Home is always open the door of that a moment, before crashing. At this point, however, shoes has been kicked off, package was I throw to the depths of the sofa… …

　　And so next time, may is so every time.

　　Used to open the computer, the trouble to listen to the shaoxing opera. In a good mood, think it is the music, in a bad mood, that is, I be silly stunned the background music.

　　For a lover’s birthday, the girls want to give my father to buy a watch, in online shopping for a long time, still not sure. Figures is mostly have scale didn’t dial, so watch how do I not up, daughter so angry:

　　”Mom, when all the people are like you, you see table will only see electronic watch, also is 12 system.”

　　”Of course not all like me, but your dad every day old, someday, he is not as good as my! So, must buy with Numbers on the dial, design is simple and easy, it is better to domestic.”

　　”Domestic mechanical or quartz? What brand do you want?”

　　”To quartz, need not everyday winding, brand to the seagull.”

　　”Gull? Never heard of.”

　　”The Internet, as a Chinese, incredibly don’t know the seagull watch?”

　　A daughter called me again:

　　”Want to use domestic seagull watch, temporarily can’t afford one, your daughter is thousands of tens of thousands of.”

　　Then, my daughter on QQ, send me a link, I open on see, isn’t it? Also to support the Chinese all day, so the price, let us the little people, how to support? A newlywed, bought a watch the seagulls, less than 200 dollars.

　　Love came back from work, said to him. See I wasn’t impressed, he looked at me and said:

　　”You are less than 200 pieces, I was monthly salary less than 120 yuan! For more than a month of wages, or to the present, not thousands of useful!”

　　”Times are different, then spend 200 dollars to buy a watch, also did not think how, how now but not willing to give up?”

　　”Don’t think that way, that was didn’t spend your money.”

　　”You don’t say you bought me a watch, to now you still love dearly!”

　　Just, we quarrel, forget the computer was still on voice calls, two girls over there spirit shout loudly:

　　”How noisy again? Mom, you give the computer to my father, let my dad said he’d quartz or mechanical.” , I put my computer to lover.

　　”I want to mechanical, don’t be too high price, didn’t I give you remit money.”

　　”Dial? Digital or graduated? Color should be black and white or grey days?”

　　”Digital calibration, color should be black and white.”

　　”Oh, is it not a watch? Things are!” I can’t help by chimed in.

　　”Dad, are you sure?”

　　”Sure, your mother rush to surf the Internet!”

　　”Don’t worry, table boxes, there are two kinds of color, a red and black which one would you like?”

　　”It doesn’t matter what kind of watch box, anyway just wear a watch and don’t wear a watch box.” And I say a word of mouth.

　　”Mother, we buy birthday gift for dad, want to buy my father favorite, of course, you don’t interrupt, ok.”

　　”What color do you like table box, what colour will! Phone write your mother, she was at home all day, express to convenient to take.”

　　Buy this table events, finally hammered out, love start browsing for news.

　　”Take headphones to you, I want to quiet for a while.”

　　”Want to quiet, what did you do for dinner?” Yeah, just and daughter discuss watch things, how to forget to do dinner?

　　You see, this want to quiet for a moment, really difficult! You can close the earth is the ear, you don’t shut the appetite of the world! Darling still cooking go to! Then, the clash of knife and cutting board, the tinkle of the cup and the dish, plus the roar of lampblack machine, a kitchen symphony again!

　　”Shut the kitchen door, noisy dead.” Lover to roar, I closed the door of the kitchen. Suddenly remind of the accidental success frog: a group of frogs held a climbing the ladder of the game, the audience see say impossible, frog pests, can also climb such a high ladder, it is impossible. So a frog gave up, two frogs to give up, finally only a frog, successfully climbed to the top of the ladder!

　　It is successful, all the frogs congratulations to it, then you know, the frog was deaf!

　　Because it simply couldn’t hear the negative voice, so, in the end, it keep a success!

　　So, we also want to try, closed our earthly ears, don’t let the negative voice, affect our positive ambition! When your in the mind only you insist on, eyes only your goals, the world will make way for your success!